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    好的转染实验(transfection reagent)就像是一件精致的艺术品,是转染效率(transfection efficiency)和细胞活性(cell viability)完美的平衡。xfect转染系列是clontech为细胞学科学家精心奉献的一只画笔,助您创作出更美好的画作!

高转染效率

  ■ 常用细胞系
  xfect transfects the following 5 most commonly transfected cell lines with very high efficiency:
 
cell type transfection efficiency
293t 97%
hek-293 93%
hela 85%
nih/3t3 82%
cho 83%
  ■ 难转染细胞系
  xfect also outperforms popular competitor transfection reagents in jurkat cells, which are notoriously difficult to transfect. xfect transfection reagent produced >40-fold higher transfection efficiency than product l. this transfection reagent can also be used to transfect human adult stem cells. we used xfect to transfect human adipose-derived stem cells (hadscs) with a transfection efficiency of 92%.

高细胞活性(very high cell viability)

  cells transfected with xfect have high viability  
 
  txfect(%) product(%)
hela 79.4%±17.9 53.2±26.7
hek-293 63.5±12.5 52.3±12.4
cho-k1 86±0.8 90.9±10.7
mcf7 51.4±10.6 29.7±21.3
 

方便使用(ease of use)

  ■ 专门准备了在血清(in presence of serum)存在条件下的简易操作方案
  ■ 优化的“傻瓜相机式操作”试剂盒包装
  ■ 专门开发了mirna/sirna/mrna以及protein转染专用的产品

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